PHYTOCHEMICAL SCREENING, CYTOTOXIC AND HYPOGLYCEMIC ACTIVITY OF METHANOLIC EXTRACT OFCITRUS SINENSISPEEL

Abstract

Orange has been traditionally used as an adjuvant in different diseases including diabetes, tuberculosis, asthma andhypertension. An examination ofthe impactsof peel extract from Citrus sinensisin hyperglycemic Albino mice uncovered the glucose lowering activity of Citrus sinensis. In this study, the methanolic extract of the fruit peel was assessed in two dosage regimen-200mg/kg and 400mg/kg, regarding the regulation of glucose inciteddiabetes mellitus. Both doses were being found to reduce the serum glucose level significantly (P value<0.0001) by 32% and 42%, respectively after 120 minutes of administration;revealing the anti hyperglycemic activity of the methanolic extract of Citrus sinensis peel. In the Brine Shrimp Lethality Toxicology study, the LC50 value of the extract was found 17.885μg/ml which indicated minimumlethal effect compared to that of standard (vincristine sulfate) which was 1.283μg/mland the phytochemical screening of the methanolic extract of the peel showed presence of different bioactive compounds like flavonoids, saponins, tannins, alkaloidswhicharehealth promoting secondary metabolites