ISOLATION AND CHARACTERIZATION TRITERPENOID COMPOUND FROM LEAVES MANGROVE PLANT (Sonneratia Alba) AND ANTIBACTERIAL ACTIVITY TEST

Abstract

Mangrove plant (Sonneratia alba) is easily found in Indonesia and has the potential of being a herb medicine. General phytochemical screening revealed the presence of flavonoid, steroid, triterpenoid, and tannin compounds. Mangrove plant variously used in ethnomedicine to treat wounds, diarrhea, and fever disease. Lup-20(29)-en-3β -ol (lupeol) compound is pentacyclic triterpenoid group. Lupeol was isolated from the methanol extract of the leaves of mangrove (S. alba). Extraction was done by maceration method using methanol 96% as solvent. Its isolation was carried out by a combination of column chromatography and combination of n-hexana, ethyl acetate, and methanol solvent. The structure was determined by analysis of IR, ¹H-NMR, ¹³C- NMR, 2D NMR and MS spectroscopies data, as well as comparison with various reference. The result of antibacterial activity test showed that isolated compound effectively inhibited the growth of these bacterial pathogens with inhibition zone 18 mm for Staphyloccocus aureus, 14 mm for Pseudomonas aeruginosa, and 13 mm for Escherichia coli. This is the first report of isolation lupeol compound from the leaves of Sonneratia alba of this species and antibacterial activity test against Staphyloccocus aureus, Pseudomonas aeruginosa, and Escherichia coli pathogen bacterial.