GC–MS ANALYSIS OF ANTIFUNGAL COMPOUNDS DERIVED FROM SOIL ACTINOBACTERIA

Abstract

This study was aimed to evaluate the antifungal property of actinobacterial strain isolated from rhizosheric soil and its compounds analysis through GC- MS. A total of 16 Actinobacterial strains were isolated and screened on the basis of their efficiency to inhibit the growth of fungal pathogen Fusarium oxysporum. The secondary metabolites from the active strain (Streptomyces werraensis KBR01) were extracted using different organic solvents and subjected to disc diffusion test to check their efficacy against fungal pathogen. The presence of active compounds was confirmed by gas chromatography-mass spectrometry (GC-MS) analysis which revealed the presence of different types of compounds like fatty acid methyl ester (6,9,12- Octadecatrienoic acid, methyl ester, 36.02%), long chain alkanes (Dodecane, 29.47%), fatty alcohol (2- pentadecanol, 13.72%), piperazinedione (2,5- Piperazinedione, 3,6-bis(2-methylpropyl), 8.28%), esters (dodecyl acrylate, 8.29%) and pyrrolizidine (Pyrrolo[1,2- a]pyrazine-1,4-dione, Hexahydro-, 2.8% and Pyrrolo [1,2-a]pyrazine-1,4-dione, Hexahydro-3-(2-methylpropyl), 1.4%). Presence of many antimicrobial compounds in the crude extract of Streptomyces werraensis KBR01 advocates the antimicrobial potency of this strain.