DEVELOPMENT OF ANALYTICAL METHOD AND ITS VALIDATION FOR SILDENAFIL CITRATE BY UV-SPECTROPHOTOMETRY

Abstract

Objective: The objective of this study is to develop a basic, delicate and precise UV-spectrophotometric method for Sildenafil citrate detection in the
bulk drugs and the drug formulations and its validation. Method: The ideal conditions were established for the investigation or analysis of the drug.
Results: The λmax of 293.2nmwas found for the Sildenafil citrate. Within, 8 to 60μg/ml concentration range the method complied with Beer's Law and
show exceptional sensitivity with linearity. Moreover, 1.012 and 3.036 were observed limit of detection and quantification, respectively. When the
absorbance versus concentration graph was plotted on the calibration curves a linear relationship was observed with the coefficient correlation of 0.99.
The observed regression coefficient (Y) of the calibration curves was found to be 0.0131x- 0.0191. The method was precise and accurate with the
experiential value of 2.0325± 0.044. The stability of the test solution was up to 48 hours. Conclusion: The proposed analytical method is simple,
economical and experimentally less time-consuming. Therefore, it will be appropriate for Sildenafil citrate analysis of pharmaceutical formulations in
bulk.