SUITABLE COMBINATION OF AUXIN AND CYTOKININ FOR CALLUS BIOMASS, TOTAL PHENOLICS, FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITIES OF LEAVES CALLI OF ACHYRANTHES ASPERA L

Abstract

Objectives: Quantitative analysis of callus biomass, total phenolic and flavonoid content and determination of antioxidant activity   were performed in 
leaves calli of Achyranthes aspera L. supplemented with various combinations of auxin and cytokinin hormone. Material and method:  Leaves calli 
with different concentrations and combination of auxins and cytokinins like 2, 4-Dichlorophenoxyacetic acid (2,4-D), Indole Acetic Acid (IAA), 
Naphthalene Acetic Acid (NAA) and 6-Benzyl Amino Purine (BAP) were initiated . During the maintenance of the calli, the sub-culturing was routinely 
done after every three week. The 60 days old callus was used to preparation of extracts. Result and conclusion: Maximum callus biomass was observed 
in leaves calli supplemented with 2,4-D (2.1 mg/l) + BAP (0.5mg/l). Highest  total phenolics  and flavonoids content  were reported in leaves calli on 
the MS medium supplemented with 2,4-D (1.5 mg/l) + IAA (0.5mg/l). Highest DPPH radical scavenging activity (85.31%), H2O2 scavenging activity 
(75.31%) and ferric reducing power (25.3µg/g) were observed in calli supplemented with 2,4-D (1.5 mg/l) + IAA (0.5mg/l). Excellent in vitro 
antioxidant activity was given by extract of callus culture on MS medium supplemented with 2,4-D (1.5 mg/l) + IAA (0.5mg/l). So they can be used as 
natural antioxidants after isolation and purification.