IN-VITRO ASSESSMENT OF THE ANTI-MICROBIAL AND ANTI OXIDANT OF MORINGA OLEIFERA AND AZADIRACHTA INDICA AGAINST BACTERIAL ENDOPHYTES: A COMPARATIVE STUDY

Abstract

medicinal plants (Moringa oleifera and Azadirachta indica) for anti-microbial and antioxidant potentials in different solvents (water and
ethylacetate). Different in vitro methods such as 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, disc diffusion methods were employed
in the study. The results revealed that Moringa oleifera has outstanding scavenging activity than A. indica, it was found that DPPH inhibition was
significantly increased with increasing amount of extract when compared to the standard control ascorbic acid. Endophytic fungi Staphylococcus
aureus and Pseudomonas were isolated from M. oleifera and A. indica. Similar approach both the leaf extract was subjected anti-microbial activity
by disk diffusion technique. The Minimal inhibitory concentration (MIC) of the extracts were determined by disc diffusion method and the results
revealed that A. indica showed promising activity against gram positive bacteria Staphylococcus aureus, Bacillus subtilis and fungi Candida; it was
found to be in effective against gram negative bacteria such as Pseudomonas, E. coli and A. niger when compared to Moringa oleifera, standard
control amoxicillin. Further work is proposed on active constituents of the plant for better lead development and its exploitation as novel bioactive
antimicrobial and antioxidant compounds.