CYTOTOXICITY OF ZINC OXIDE NANOPARTICLES IN HELA CANCER CELL AND ITS EFFECT TO APOPTOSIS VIA P53 AND CASPASE PATHWAY

Abstract

Cancer is the second causing death diseases in the world. Nanomedicine emerged as a promising alternative for the treatment of cancer. HeLa is the 
very commonly employed model cell line for studying human cellular and molecular biology. Nanoparticles are a special institution of substances with 
precise capabilities and significant applications in lots of biomedical fields. Zinc oxide nanoparticles (ZNO) showed interested biocompatibility 
properties such selective accumulation in tumor cells. In this study, the work Zinc oxide nanoparticles are set up through Sol-Gel approach. The 
combination of nanoparticles distinguished through the utilization of DLS and TEM. In- vitro anticancer activity of Zinc oxide nanoparticles towards 
Hela cancer cell line  utilizing various parameters had been studied .  To exert cell growth arrest against Hela cancer cell lines had been determined  by 
using Zinc oxide  nanoparticles, the proficiency antiproliferative of ZnO NPs is because of cell dying and inducing apoptosis that affirmed by  the usage 
of acridine orange-Ethidium bromide dual  staining , Genotoxicity examine through  RT- PCR analysis turned into done to detect  the gene expression 
of Caspase-8, Caspase-9, and P53. In this study, we can suggest that ZnO NPs may well be utilized  to supply  a new drug restitution a chemotherapy 
drug and an  enormous medical applications .