SIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF DOXYCYCLINE MONOHYDRATE AND ORNIDAZOLE IN SYNTHETIC MIXTURE

Abstract

A simple UV spectrophotometric absorption ratio method has been developed for the simultaneous determination of Doxycycline monohydrate and Ornidazole in synthetic mixture. This method uses the ratio of absorbances at two selected wavelengths, one which is λ max of Ornidazole (311.4 nm) and other being an Isoabsorptive point of both drugs (286.6 nm) in methanol. The linearity was obtained in the concentration range of 3-27 μg/ml for both Doxycycline monohydrate and Ornidazole. The results of analysis have been validated statistically and also by recovery studies according to ICH guidelines. The method was successfully applied to synthetic mixture because no interference from the mixture excipients was found. The Method was found to be simple, sensitive, accurate and precise and was applicable for simultaneous estimation of Doxycycline monohydrate and Ornidazole in synthetic mixture.