ALTERATION IN UTERINE HISTOARCHITECTURE AND UTERINE PROTEIN FOLLOWING BUTYLPARABEN EXPOSURE IN ADULT MICE

Abstract

Environmental chemicals with estrogenic properties are widely found in products widely used by human. Butylparaben (BuPben), a chemical with estrogenic activities is found to have adverse effects on the female reproductive tract. Adult mice were exposed to10 mg, 50 mg and 100mg dose of BuPben for 7 and 21 days. In the study 0.001 mg/Kg body weight 17βestradiol was used as positive control and Olive oil and olive oil + ethanol (1:10) was used as control and vehicle control respectively.Subcutaneous exposure to 50 mg/kg body weight (bw)and 100 mg/kg bw of BuPbenisfound to cause significant increasein uterine weight when compared to control and vehicle control group in adult mice. In this study 50mg and 100 mg dose ofBuPben, similar to 17 βestradiol(E2)(used as positive control) showed increase inthickness of myometrium and endometriumwith adverse effects on uterine histology. Increase in number of uterine glands in also reported by all the doses of BuPbenincluding 10 mg, 50 mg and 100mg when compared to control groups, along with significant decrease in concentration of serum estradiol and serum progesterone. Increase in uterine protein was also found in the study following BuPbenexposure.Thus BuPbenexposure adversely affects uterus in mice.This adverse effect of the chemical describes estrogenic activity of the chemical which may lead to reproductive failure in human and animals which gets exposed to the chemical