COMPARATIVE STUDY ON THE SYNERGISTIC ACTION OF DIFFERENTIALLY SYNTHESIZED COPPER NANOPARTICLES WITH ESCHERICHIA COLI AND STAPHYLOCOCCUS AUREUS

Abstract

The synthesis of metal nanoparticles has received much attention due to their wide range of applications. Copper nanoparticles, due to their interesting
properties, low cost preparation and many potential applications in catalysis, microbial activity, cooling fluid or conductive inks, have attracted a lot
of interest in recent years. This research is carried out to compare the stability and antibacterial activity of the biologically and chemically synthesized
copper nanoparticles of two different nanometer ranges. In the chemical synthesis of copper nanoparticles copper sulphate is used as the precursor,
ascorbic acid (natural vitamin C) was employed as a reducing agent and Polyethylene glycol (PEG) is used as a capping agent whereas in biological
synthesis the high medicinally valued Aqueous Root bark extract of Sansevieria trifasciata acts both as a reducing as well as the capping agent. The
synthesized Cu-NPs were analyzed by UV-Vis spectroscopy, X-ray diffraction, scanning electron microscopy and energy-dispersive X-ray
spectroscopy measurements were taken to investigate the size, structure and composition of synthesized Cu Nano crystals, respectively. Antibacterial
effect against different strains of microbial species and the zone of inhibition of growth of microbes are also investigated.