EXPERIMENTAL EVALUATION OF ANALGESIC EFFECT OF CLOVE OIL WITH COMPARISON OF TRAMADOL IN MICE

Abstract

Background: Pain, the most common reason for patients, is an unpleasant sensation. It is generally caused by 
inflammatory reaction in the tissue or tissue damage and produced emotional reaction. Clove oil is an 
important aromatic spice, which belongs to the Myrtaceae family, contains high number of phenolic 
compounds with several biological activities, including analgesic, anticonvulsant, antibacterial, antifungal, 
insecticidal and antioxidant properties. Eugenol is the major compound in Clove essential oil. It is used as 
a local antiseptic and analgesic and anesthetic. Aim: To study the analgesic activity of clove oil in comparison 
to tramadol in mice. Methods: This experimental study was done in central animal house, Department of 
Pharmacology, Gandhi medical college, study duration from 18th august 2022 to 1st october . Three months old 
Male albino mice(n=15) were divided into three groups: 1) control group treated with normal saline, 2) standard 
group treated with inj.Tramadol 50mg/kg(Zydus corza zydus health are Ltd)., 3) Test group treated with 
Inj.clove essential oil 0.1 ml/kg (Dwibhashi bapamma Ayurvedic Nilayam Pvt.Ltd.) 15 min before testing in hot 
plate. Analgesic effect of clove oil and Tramadol had been seen with Hot plate method as a basic method to 
induce their respective doses. Repeated measures of ANOVA with Post-hoc Tukey’s HSD test were used for 
statistical analysis. Results: Repeated measures of ANOVA with Post hoc Tukey’s HSD test showed that 
maximum analgesic effect in group treated by 10% Clove essential oil was significantly higher (P value<0.05)  
than Normal saline group and similar to Tramadol group. Conclusion: Present study results showed that Clove 
oil may possibly act as analgesic agent from heat with stimulates the nociceptive mechanism. Clove essential oil 
has analgesic action similar to Tramadol in mice using hot plate test.