IDENTIFICATION OF PHYTO CONSTITUENTS AND DEVELOPMENT OF TLC AND HPTLC FINGER PRINT PROFILES FOR EPALTES PYGMAEA DC.

Abstract

Objective of the present work was to establish of the finger print profile of five isolated compounds namely β-amyrin acetate, β-amyrin, lupeol, 
stigmasterol, β-sitosterols in Epaltes pygmaea DC using thin layer chromatography and high performance thin layer chromatography (TLC/HPTLC) 
techniques. β-amyrin acetate, β-amyrin, lupeol, stigmasterol, β-sitosterols, n-hexane, chloroform, ethyl acetate and ethanol extract of the Epaltes 
pygmaea whole plant were applied on a TLC plate, developed using Toluene: Ethyl acetate as solvent system and visualized under UV 254 nm and 
366nm for photo documentation. The HPTLC finger printing of the n-hexane, chloroform, ethyl acetate, ethanol extracts were developed in the visible 
light at 520 nm. Presence of β-amyrin acetate, β-amyrin, lupeol, stigmasterol, β-sitosterols were confirmed by UV spectra comparison of compound 
and respective peaks of similar Rf in extract. TLC and HPTLC finger print profile one of the useful tool in compound identification and authentication 
in medicinal plants.