ANTIBACTERIAL ACTIVITY OF HUMPED BLADDERWORT (UTRICULARIA GIBBA) EXTRACTS RESULTING FROM MULTISTAGE EXTRACTION METHOD

Abstract

The purpose of this research was to identify antibacterial activity of humped bladderwort (Utricularia gibba) with multistage extraction utilizing swamp 
plants as a natural antibacterial. The parameters observed were tannins and phenol by quantitative analysis. The yield of extracts with solvent n-hexane, 
ethyl acetate and ethanol  were 0.50%, 1.66%, 3.27%, respectively. Quantitative testing of tannins from humped bladderwort (Utricularia gibba) extract 
were 215.12 ppm from the extract with n-hexane solvent, 221.86 ppm from the extract with ethyl acetate solvent and 225.70 ppm from the extract with 
ethanol solvent and testing of phenol levels were 108 ppm from the extract with n-hexane solvent, 122 ppm from the extract with etyl acetate solvent 
and 152 ppm from the extract with ethanol solvent. The antibacterial activity indicate that the extract of humped bladderwort  (Utricularia gibba) can 
inhibit the bacterial of Bacillus subtilis, Salmonella thypimurium and Escerichia coli. Inhibitory zone was highest at concentration of 2.000 ppm using 
an ethyl acetate solvent.