EVALUATING THE RELATIONSHIP BETWEEN GLYOXALASE 1 AND SERUM LEVELS OF MAGNESIUM, MANGANESE AND SELENIUM IN INDIVIDUALS WITH DIABETES MELLITUS

Abstract

Background: Glyoxalase-1 is an erythrocyte enzyme that is part of the glyoxalase system. It aids in the catabolization of 
methylglyoxal and is also a precursor for AGEs (advanced glycation end products). Elevated levels of AGEs and 
methylglyoxal result in diabetic complications that are impacted by Glyoxalase levels.  
Aim: The objective of this study was to assess the levels of serum magnesium (Mg), manganese (Mn), and selenium (Se) in 
different groups defined by the length of diabetes mellitus.  
Methods: The present study 252 participants with diabetes mellitus between the ages of 30 and 70 were evaluated and 
compared the concentration of different micronutrients, namely manganese, selenium, and magnesium. For biochemical 
investigations, the level of Glycated haemoglobin (GlyHb) was measured for each subject with lipid levels. An entirely 
automated analyzer and the immunoturbidity enhance enzymatic (IEE) approach were used to analyse glycated 
haemoglobin. ELISA was used to measure the levels of Glyoxalase-1. One-way ANOVA, the independent t-test, and the 
chi-square test were used in the statistical analysis of the collected data. 
Results: glyoxalase-1 levels were strongly correlated with glycated haemoglobin, selenium, and magnesium levels (p
values of 0.007, 0.02, and <0.001, respectively), whereas manganese levels were not significantly correlated (p=0.72). 
In diabetic patients, glyoxalase-1 is a good predictor of inadequate glycemic control. In diabetic people, there is a 
correlation between high levels of glycoxalase and the premature onset of problems. Serum magnesium, selenium, and 
manganese concentrations all show a decline as diabetes duration increases. 
Results: Groups I and II had mean diabetes durations of 2.22±0.94 years and 6.83±1.92 years, similar mean BMIs 
(24.3±3.1 and 23.6±3.5 kg/m2, p=0.74), Glycated haemoglobin for laboratory studies was 8.9±2.3 and 9.9±2.5 (p=0.02) 
respectively. the levels of triglycerides, total cholesterol, HDL (high-density lipoproteins), LDL (low-density lipoproteins), 
and VLDL (very low-density lipoproteins) were similar in both groups. Glyoxalase-1 levels were strongly correlated with 
glycated haemoglobin, selenium, and magnesium levels (p-values of 0.007, 0.02, and <0.001, respectively), whereas 
manganese levels were not significantly correlated (p=0.72).