ANALYSIS OF IN VITRO ANTIOXIDANT AND ANTIMICROBIAL POTENTIAL OF Putranjiva roxburghii Wall.

Abstract

Putranjiva roxburghii Wall. has been historically credited with a significant role in the traditional Unani and Ayurvedic systems of medicine. The 
present study was an attempt for evaluation of antioxidant and antimicrobial efficacy of the methanolic extracts from the bark and seeds using various 
in vitro assays. The antioxidant activity of the extracts was estimated using the DPPH radical scavenging assay and ferric reducing antioxidant power 
assay. Extract concentrations in the range of 50 – 500 µg/ml for bark and 200 – 2000 µg/ml for seed were assayed to determine the IC50 values in DPPH 
assay. For the evaluation of Ferric reducing capacity, concentrations in the range 50 – 1000 µg/ml were compared. The extracts were also analyzed for 
antibacterial and antifungal activity against Escherichia coli, Staphylococcus aureus, Aspergillus niger and Candida albicans. The results suggested 
that bark has significant antioxidant and antimicrobial properties than the extract of seed. The IC50 for bark and seed extract were 129.47 µg/ml and 
1978.7 µg/ml respectively. Both the DPPH and ferric reducing assays suggested a dose dependent increase in extract efficiency to scavenge and to 
reduce the free radicals. The antimicrobial activity suggested that bark extract could inhibit bacterial (IZ 10 – 12 mm) and fungal growth (IZ = 12 – 14 
mm) at 1000 µg/ml concentrations.