STUDY OF ANTIOXIDANT ACTIVITY WITH REDUCTION OF DPPH RADICAL AND XANTHINE OXIDASE INHIBITOR OF THE EXTRACT OF RUELLIA TUBEROSA LINN LEAF

Abstract

Ruellia tuberosa L belongs to Acanthaceae family. In traditional medicine has been used as diuretic, antidiabetic, antipyretic and antihypertensive, and it also recently been incorporated as a component in a herbal tea in Taiwan. This research aims to determine the antioxidant activity of R. tuberosa leaf extract. Extraction of R. tuberosa leaf by stratified maceration method using dichloromethana and methanol. Methanol extract was partitioned by using ethyl acetate and n-butanol. Antioxidant activity was tested by reduction of  DPPH radical method and inhibition of the xanthine oxidase.