SIMULTANEOUS ESTIMATION OF MULTICOMPONENT FORMULATIONS BY UV-VISIBLE SPECTROSCOPY: AN OVERVIEW

Abstract

UV-visible spectroscopy, a simple, rapid, precise and highly accurate method for quantitative estimation is in great use now a day. The basic principle behind this technique is that the amount of light absorbed is proportional to the concentration of analyte. Simultaneous equation is applicable for the estimation of those drugs where the spectra of drugs overlap properly whereas multi-component analysis can be applied on any degree of spectral overlap provided that two or more spectra are not similar exactly. Quantitative estimation is necessary before introduction of any drug into the market as either concentration is more in formulation can cause toxicity problem or if concentration is found less, then formulation may not be effective in prescribed dose.