A STUDY OF OCCURRENCE OF ANTIBIOTIC RESISTANCE REPORTED AGAINST KLEBSIELLA SPP. IN A TERTIARY CARE HOSPITAL IN ASSAM, INDIA

Abstract

A prospective study was conducted in the Department of Microbiology, AMCH (Assam Medical College and Hospital), where pus, urine, blood, sputum and wound swab samples were collected of patients from new-born to 85 years of age and cultured on MacConkey Agar and Blood agar media plates at 37⁰ C for 18-24 hrs.(overnight). A total of 100 samples positive for Klebsiella spp. were collected. Bacteria were identified by their culture, morphological and bacteriochemical characteristics using standard microbiological methods. Antibiotic sensitivity was tested using conventional disc-diffusion method by Kirby- Bauer technique using Mueller-Hinton Agar plates. A total of 100 Klebsiella spp. positive samples were studied with 9 antibiotics as mentioned later. The data on percentage antibiotic resistance out of 100 samples were Amoxycillin-Clavulanic acid=75%, Ceftriaxone=69%, Cefotaxime=65%, Piperacillin- Tazobactam=57%, Amikacin=47%, Gentamycin=46%, Ciprofloxacin=41%, Ofloxacin=32% and Imipenem=0%.Increased antibiotic resistance of Klebsiella spp .as seen in the study conducted increases concern over formulation of proper, national antibiotic policy and its proper implementation along with issues of “Antibiotic Stewardship”.