THE ASSOCIATION OF RESISTIN GENE –420C/G (RS1862513) SINGLE NUCLEOTIDE POLYMORPHISM WITH TYPE TWO DIABETES MELLITUS IN IRAQI POPULATION

Abstract

Background: Worldwide studies have shown polymorphisms within the resistin gene like–420C/G (rs1862513) to verify the effect of these 
polymorphisms in the occurrence of  T2DM. Aim: To study the association of –420C/G  with T2DM in Iraqi population. Methods: The study consisted 
of 400 T2DM patients and 400 healthy control individuals. Genotyping of  –420C/G  polymorphism  is achieved  by PCR–RFLP. DNA was extracted 
from whole blood and genotyping was carried out by specific primers to amplify fragments for digestion with restriction enzyme. Bbs I restriction 
enzyme used for   digestion   followed by electrophoresis on agarose gel. Various statistical analyses were used to analyze the data. Results: The 
genotype and allele frequencies of rs1862513 SNP revealed insignificant differences among the  homo (GG) (OR=  1.42  ,CI 95%;0.95-2.11;P=0.08), 
hetero (CG) (OR= 1.07  CI 95%; 0.78-1.45, P= 0.7), dominant (OR = 1.42, CI 95%;  0.96-2.11, P= 0.08) and recessive (OR = 1.37, CI 95%; 0.96
1.98, P= 0.08) models. Furthermore, the minor allele frequency (G) was insignificantly increased (P= 0.21) in T2DM when compared with the control 
group. Conclusion: Resistin gene (rs1862513) polymorphism was exhibited non-significant  association with T2DM   in Iraqi  population.