IN VITRO ANTIBACTERIAL ACTIVITIES OF LAWSONIA INERMIS LEAF EXTRACTS

Abstract

To know the in vitro antibacterial activities of n-hexane, chloroform, ethyl acetate, benzene, acetone and acetronitrile extracts of the leaves of Lawsonia inermis(Family- Lythraceae), present study was conducted. All the extracts were used at 50mg/ml, 100mg/ml and 300mg/ml concentrations. For the present study Staphylococcus aureus, Bacillus subtilis and Escherichia coli were used. Against Bacillus subtilis, the ethyl acetate extract was more potent than tetracycline (25µl/ml), which was used as a positive control. On the other hand, the chloroform extract and tetracycline more or less produce equal zone of inhibition against that strain. n-hexane, chloroform, ethyl acetate, acetone and acetonitrile extracts were effective against all the strains used in the study. All the extracts of Lawsonia inermis, which were having antibacterial activities, produced such activities in a dose dependent manner. For the study dimethylsulfoxide was used as negative control.