SYNTHESIS, CHARACTERIZATION AND ANTI MICROBIAL STUDIES OF AgNP’S USING PROBIOTICS

Abstract

Recent researches speak about the utilization of biological systems especially enterobacteria to synthesize nanoparticles is a novel approach. The present study demonstrates an eco-friendly and low cost protocol for synthesis of silver nano particles using the cell free filtrate of Lactobacillus bulgaricus when supplied with aqueous silver ions. Characterization of silver nanoparticles was done by UV-Visible absorption spectroscopy shows maximum absorption at 420 nm, Transmission electron microscope (TEM) revealed the formation of spherical nanoparticles with size ranging between 40 – 50nm, X-Ray diffraction (XRD) pattern shows spectrum, which reveals the FCC structure of silver nanoparticles. Energy dispersive spectroscope (EDS) analysis was done using SEM instrument equipped with thermo EDS attachment shows the optical absorption peak at 3kev. Microbial nanoparticles showed very strong inhibitory action against Staphylococcus aureus, Staphylococcus albus, & Pseudomonas aeruginosa.