PHYTOCHEMICAL SCREENING AND EVALUATION OF SEED EXTRACT OF PSORALEA CORYLIFOLIA LINN. BY GC-MS AND FT-IR SPECTRUM ANALYSES

Abstract

Background: Psoralea corylifolia Linn (Fabaceae), a medicinally important plant, indigenous to tropical and 
subtropical regions of the world and is reported in the Indian Pharmaceutical Codex, Chinese, British and American 
Pharmacopoeias and in different traditional systems of medicine, such as, Ayurveda, Unani and Siddha.  
Objectives: The present study was conducted to investigate the phytochemical composition and functional groups of P. 
corylifolia seeds.  
Methods: The shade dried seed materials were subjected to extract. The methanolic extract was subjected to gas 
chromatography-mass spectroscopy (GC-MS) and Fourier Transform Infra Red Spectroscopy (FT-IR) analyses.  
Results: A total of forty compounds were identified from GC-MS analysis and the predominant compounds were 
identified as an Isopsoralen (57.45%) at the retention time of 20.967, Di-hydroxy Coumestan (12.96%) at the retention 
time of 18.299, Isobavachin (11.52%) at retention time of 17.654, Beta Caryophyllene Oxide (2.94%) at retention time 
of 15.240 and 1,7, Cyclo Octene (2.68%) at the retention time of 22.952. Furthermore, FT-IR analysis of P.corylifolia 
proved the presence of Phenols, Alkanes, Aldehydes, Alcohols, Isocynate, Aromatics, Carboxylic acid, Aliphatic 
amines and amines which shows major peaks(cm-1) at 3412.14, 2949.39, 2842.98, 2522.06, 2075.82, 1638.46, 1512.99, 
1453.39, 1412.74, 1268.98, 1111.97, 1052.14, 1032.11, 1018.75 and 667.24 respectively.  
Conclusion: The plants studied here can be seen as a potential source of useful drugs. Further studies are going on these 
plants in order to isolate, identify, characterize and elucidate the structure of the bioactive compounds and to develop 
novel phytochemical marker to identify the medicinally important plant.