ISOLATION AND IDENTIFICATION OF PIGMENT MOLECULES FROM LEAVES OF PROSOPIS JULIFLORA

Abstract

Crude pigments were extracted from fresh leaves of Prosopis juliflora by use of ethanol. It was subjected to repeated column chromatography for further purification, with silica as stationary phase and petroleum ether, ethyl acetate and methanol as mobile phase. At each step of separation purity was checked by thin layer chromatography and HPTLC analysis. Identification of the compound was done by UV-visible spectrometry and ¹H NMR technique and then comparing these data with the data present in the chemical libraries. The pigments isolated from the leaves of P.juliflora., include, two carotene, three xanthophylls and two pheophytin.