VALIDATED RP-HPLC METHOD FOR ANALYSIS OF MILNACIPRAN IN BULK AND IN FORMULATIONS

Abstract

A rapid, simple and validated reversed-phase high-performance liquid chromatographic method has been developed for analysis of Milnacipran in tablet dosage form. Milnacipran was separated on an Inertsil ODS analytical column with a 40:60 (v/v) mixture of Methanol and Octane sulfonic acid buffer (containing 0.05%w/v Octane sulfonic acid with 0.025%v/v Trifluoro acetic acid) as mobile phase at a flow rate of 1.0 mL min. The effluent was monitored by UV detection at 220 nm. Calibration plots were linear in the range of 10 to 50 μ g mL and the LOD and LOQ were 0.536 and 1.624 μ g mL, respectively. The high recovery and low relative standard deviation confirm the suitability of the method for routine quality control determination of Milnacipran in capsules.