MICROBIOLOGICAL ASSAY FOR VITAMIN B

Abstract

Micronutrients added to foods are analyzed using various procedures depending on their nature and properties. The microbiological assays are better than chemical method because any suitable change in vitamin molecule which may not be detected by chemical method will be revealed by change in microbial activity. The microbiological assay of vitamins is based upon the comparison of the stimulation of growth of bacteria by measured concentration of vitamin with that produced by known concentration of standard preparation of Vitamin having known activity. Microbial assay of vitamin B-12, Calcium Pantothenate, Niacinamide can be done by individual transmittances method, which observes transmittance by spectrophotometer. The microbiological assay of antibiotics and vitamins were studied by using various strains of microbes.