ANTI-INFLAMMATORY ACTIVITY OF SESBANIA SESBAN (L) MERR.

Abstract

The current study was conducted to obtain three different extracts from Sesbania Sesban (L) MERR. leaves. To achieve crude extracts, leaves were dried in shade under normal environmental conditions and then subjected to size reduction to coarse powder. Performing successive hot soxhlet extraction using petroleum ether (60-80⁰), Chloroform and methanol. Then study detail phytochemical investigation and pharmacological screening of all extracts. Methanolic extract showed significant activity. Methanolic extract was subjected to thin layer chromatography, which showed three spots i.e 1, 2 and 3. The methanolic extract was then subjected to column chromatography for separation and isolation of the constituents present in it. The constituents were collected in fractions. The elution of the column yielded three compounds. All the three separated constituents were subjected to evaluation of anti-inflammatory activity. Constituent no. 2 showed significant activity.