DEVELOPMENT AND VALIDATION OF THE RP-HPLC METHOD FOR DETERMINATION OF BENZIMIDAZOLE CARBAMATES IN THE PRESENCE OF CYCLODEXTRINS

Abstract

Liquid chromatography is the universal method to determine drugs in biological matrices and pharmaceutical products. Different strategies have been developed in order to improve the aqueous solubility and bioavailability of benzimidazoles, such as the complexation with cyclodextrins. Although extensive studies on cyclodextrins have been performed, studies on the impact of cyclodextrins on the chromatographic process are scarce. The objective of this study was to develop and validate a suitable HPLC method for the quantification of benzimidazoles carbamates (albendazole and fenbendazole) in the presence of cyclodextrins (\bete-cyclodextrin and hydroxypropyl-\bete-cyclodextrin). The retention time of the benzimidazoles and their metabolites decreases in the presence of cyclodextrins. The formation of inclusion complexes between drugs and cyclodextrins can explain the change in retention times. Hydroxypropyl-\bete-cyclodextrin decreased the retention time of the solute in a more pronounced way than \bete CD. This phenomenon can be attributed to the greater hydrophilicity of Hydroxypropyl-\bete-cyclodextrin, which promotes the formation of more soluble complexes and, therefore, with less affinity for the stationary phase.