UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF NEW DRUG, DACLATASVIR DIHYDROCHLORIDE

Abstract

Daclatasvir dihydrochloride (DCH) is a new drug gained its FDA approval on July 24, 2015 for treatment of hepatitis C. As there are no reported UV spectrophotometric methods for estimation of daclatasvir dihydrochloride, the present work was aimed at development of accurate and precise spectrophotometric method for its estimation by absorbance maxima method. The working standard solution of 10 μg/ml was scanned in the wavelength range of 400-200 nm. Absorption maximum, λmax was found at 214 nm. Calibration curve was obtained with good correlation coefficient value of 0.986. Linearity was observed in concentration range of 2-12 μg/ml. Method accuracy was revealed by recovery studies obtained in between
99.95 and 100.09.