DEVELOPMENT AND VALIDATION OF A NOVEL HPTLC METHOD FOR SIMULTANEOUS ESTIMATION OF EMBELIN AND CATECHIN

Abstract

A new simple, precise, rapid and selective high-performance thin-layer chromatographic (HPTLC) method have been developed for the simultaneous determination of embelin and catechin in herbal formulations containing cashew leaves and amla fruit powder. Two herbal formulations were prepared in-house as anti-oxidant and anti-bacterial medicine, one of which is tablet and second is cream. These formulations were analyzed by developed and validated HPTLC method for two main phytoconstituents (Catechin and Embelin). This method shows well resolved peak of embelin and catechin. The results obtained in validation assays indicate that the method is linear, accurate, précised and robust. This developed and validated method is well applied for the analysis of the in-house formulations, which in-turn can be applied in large scale. This routine analysis will take our herbal medicine to the global level.