RP-HPLC METHOD FOR SIMULTANEOUS DETERMINATION OF LOSARTAN AND CHLORTHALIDONE IN PHARMACEUTICAL DOSAGE FORM

Abstract

A simple, precise, accurate and reproducible high performance liquid chromatography method was developed for the simultaneous quantitative determination of antihypertensive drugs Losartan and Chlorthalidone from their combined drug product. The separation was achieved by a simple isocratic method using phenomenex C18 column. The mobile phase contains a mixture of acetonitrile and water in the ratio 80:20 percentage v/v at a flow rate of 1.0 ml/minute and the column was maintained at normal temperature. The detector wavelength was 284 nanometer. The retention times of Losartan and Chlorthalidone were 1.72 minutes and 2.64 minutes respectively with a total runtime of 10 minutes. The described method was validated with respect to system suitability, specificity, linearity, precision and accuracy. A low percentage RSD indicated the method was accurate and precise and can be used for the quantification of these drugs in pharmaceutical dosage forms and biological fluids.