RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CILNIDIPINE AND TELMISARTAN IN COMBINED PHARMACEUTICAL DOSAGE FORM

Abstract

A specific, accurate, precise and reproducible RP-HPLC method development validation for simultaneous estimation of cilnidipine and telmisartan in combined pharmaceutical dosage form. The proposed HPLC method utilizes Youglin C18 column (250 mm x 4.6 mm) 5 µm particle sizes and mobile phase consisting of Acetonitrile: 0.05% ortho phosphoric acid (60: 40) at a flow rate of 0.7 ml/min with UV detection 236 nm. The retention time of cilnidipine and telmisartan were found to be 4.650 min and 8.050 min respectively. The method was validated in terms of accuracy, precision, Linearity and robustness and was successfully applied for the determination of investigated drugs in combined dosage form.