BIOANALYTICAL METHOD DEVELOPMENT: AN UPDATED REVIEW

Abstract

In this review techniques used for the Bioanalytical Method Development for the extraction of analyte present in plasma contain K2EDTA as an
anticoagulant from LC/MS/MS or HPLC is discussed. The extraction is performed by using different methods i.e. Liquid-Liquid Extraction, Solid
Phase Extraction and Protein Precipitation. This literature mainly focused on the benefit of LC/MS/MS and advantages and disadvantages of different
methods used for extraction of analyte in human plasma. This review article will give information to all those researchers who were engaged in
bioanalytical research work by LC/M/MS.