COMPARATIVE SEM STUDY ON TETRACYCLINE HYDROCHLORIDE ROOT CONDITIONING: THE EFFECTS OF DIFFERENT CONCENTRATIONS AND APPLICATION TIMES

Abstract

Smear layer removal and collagen fiber exposure may improve periodontal treatment and regeneration. This in vitro study assessed smear layer removal and morphological changes (collagen fiber exposure) after tetracycline hydrochloride (TTC) application on root surfaces using different concentrations and application times by scanning electron microscopy (SEM). 672 samples were divided into eight groups: a control (distilled water) and seven different TTC concentrations were applied at doses of 10, 25, 50, 75, 100, 125 and 150 mg/ml. The TTC application was performed in all groups at three different periods of conditioning (1, 2, and 3 minutes) by burnishing method. A previously trained, calibrated examiner evaluated photomicrographs.Statistical analysis was performed using the Fischer Exact test. Tetracycline hydrochloride concentrations between 50 mg/ml and 150 mg/ml and all application periods used, showed effective root surface changes. The concentrations of 50 mg/ml, 75 mg/ml and 100 mg/ml at 3 and 5 minutes showed most effective smear layer removal. Most effective morphological root surface changes were seen at 100 mg/ml TTC solution at 5 minutes. The concentrations of 50 mg/ml, 75 mg/ml and 100 mg/ml during 5 minutes were the most effective for smear layer removal and 100 mg/ml TTC solution at 5 minutes showed most effective morphological root surface changes.