PROFILING OF BIOACTIVE COMPONENTS PRESENT IN Ziziphus mauritiana Lam FOR IN-VITRO ANTIOXIDANT AND IN-VIVO ANTI-INFLAMMATORY ACTIVITIES

Abstract

The aim of the present study is to analyze the methanol extract of Ziziphus mauritiana Lam leaves for its chemical composition using gas
chromatography – mass spectroscopy (GC- MS) analysis correlating in-vitro anti-oxidant, in vivo-anti-inflammatory activity and to determine the
total phenolic & flavonoid contents. The processed plant material was extracted using methanol. Phytochemical profiling of the extract revealed
presence of flavonoids, terpenoids, alkaloids and glycosides. The antioxidant activity of the extracts was determined using 2,2ꞌ-Diphenyl-1-
picrylhydrazylhydrate (DPPH), 2,2′-azino bis-3-ethylbenzothiazoline-6 sulfonic acid (ABTS) and reducing power assay. Anti-inflammatory
activity was evaluated by cotton pellet granuloma in wistar rat model. Twenty three phytoconstituents identified using GC-MS analysis out of that
nine were familiar with earlier reports on its pharmacological background. The IC50 values for the DPPH activity was 7.64μg, for ABTS activity
43.79 μg and for reducing power assay 26.35μg. The methanol extract of Z. mauritiana leaves showed a significant reduction (p < 0.001) in the
cotton pellet granuloma in a dose-dependent with a maximum that reaches at 500 mg/kg. Hence, flavonoids and phenolic compounds were associated
with the antioxidant and anti-inflammatory activities observed for Z. mauritiana. The effect of the extract might be due to the presence of active
phytomolecules such as n – hexadecanoic acid and L-(+)-ascorbic acid 2, 6-dihexadecanoate. From these results it was clearly evident that the
phenolic and flavonoid compounds from Z. mauritiana leaves are responsible for anti-inflammatory and antioxidant activities.