THE ANTIMICROBIAL EFFECTS OF SILVER NANOPARTICLES ON THE MULTIDRUG-RESISTANT KLEBSIELLA CLINICAL ISOLATES

Abstract

The aim of the present work is to study the properties of Silver nanoparticles prepared by chemical reduction method and their antimicrobial effects on the multidrug-resistant (MDR) Klebsiella clinical isolates. Silver nitrate was taken as the metal precursor and sodium citrate as a reducing agent. The Ultraviolet–visible (UV-Vis) absorption spectroscopy used for monitored the formation of the silver nanoparticles. The average size and morphology of silver nanoparticles were determined by transmission electron microscopy (TEM) and the diameter of particle was in the range of 5 to 45 nm and spherical in shape. The combined effect of these nanoparticles with Tetracycline, Doxycycline and Ampicillin was investigated and showed enhanced activity against MDR Klebsiella isolates. The maximum synergistic effect was observed for Ampicillin and Tetracycline with AgNPs.