ASSESSMENT OF PHARMACOKINETIC PARAMETERS OF PHARMACODYNAMICALLY ACTIVE CURCUMIN GEL FOR ASSESSMENT OF CAUSE FOR COMPLETE RECOVERY OF RHEUMATOID ARTHRITIS

Abstract

Curcumin gel prepared with solid lipid nanoparticles (SLN’s) revealed its high ex-vivo characteristics and pharmacodynamically effective against
collagen induced rheumatoid arthritis (RA) for rats. Hence the present research work was aimed to assess the reason for compl ete disappearance of
swellings appeared due to RA. Therefore, pharmacokinetic parameters were estimated by self-developed HPLC method of analysis. Two groups were
treated with topically applied gels. One group with curcumin gel made with SLN’s and other group with gel containing pure curcumin.
Pharmacokinetic parameters of gel with SLN’s of curcumin were found to be Cmax, t1/2, and AUC0-t 18.95 µg/ml, 5.833 hours and 416.75 µg/ml/hour
respectively and Cmax, t1/2, and AUC0-t of pure curcumin gel were found be 8.786 µg/ml, 4.9691 hours and 253.6 mg/ml/hour respectively. Tmax for
both formulations was found to be 2.018 hours and 2.6492 hours respectively. The amount of drug absorbed is higher with the gel containing SLN’s
of curcumin (Cmax = 18.95 µg/ml) compared to pure curcumin gel (Cmax = 8.786 µg/ml). It also shows that gel containing SLN’s of curcumin which
has regression value of 0.973 when compared with ex-vivo permeation studies. Hence the gel exhibits maximum accumulation of drug at site of action
like swollen joints as well as reasonable percent availability of curcumin in blood plasma leading to effective recovery of rheumatoid arthritis.